Capturing genetic variability and selection of traits for heat tolerance in a chickpea recombinant inbred line (RIL) population under field conditions
To assess the genetic variability and identify heat responsive traits in chickpea, a set of 296 F8–9 recombinant inbred lines (RILs) of the cross ICC 4567 (heat sensitive) × ICC 15614 (heat tolerant) was evaluated under field conditions at ICRISAT, Patancheru, India.
Accelerating genetic gains in legumes for the development of prosperous smallholder agriculture: integrating genomics, phenotyping, systems modelling and agronomy
To achieve faster genetic gains in legumes in rainfed conditions, this review proposes the integration of modern genomics approaches, high throughput phenomics, and simulation modelling to support crop improvement that leads to improved varieties that perform with the appropriate agronomy.
High-density genetic map using whole-genome resequencing for fine mapping and candidate gene discovery for disease resistance in peanut
This paper evaluates efforts to generate whole-genome resequencing data on a recombinant inbred line population, develop a SNP‐based high‐density genetic map, and conduct fine mapping, candidate gene discovery and marker validation for early leaf spot, late leaf spot, and Tomato spotted wilt virus in peanut.
Development and evaluation of high-density SNP array (Axiom®CicerSNP Array) for high resolution genetic mapping and breeding applications in chickpea
Screening of whole-genome resequencing data from 429 chickpea lines using the high throughput SNP genotyping platform ‘Axiom® CicerSNP Array’ identified 4.9 million SNPs, from which a subset of 70,463 high-quality nonredundant SNPs was selected using different stringent filter criteria.
Toward the sequence-based breeding in legumes in the post-genome sequencing era
This this article, the authors propose a sequence-based breeding approach which includes use of independent or combination of parental selection, enhancing genetic diversity of breeding programs, forward breeding for early generation selection, and genomic selection using sequencing/genotyping technologies.
Genetic imprints of domestication for disease resistance, oil quality, and yield component traits in groundnut
This article analyses how the difference in ploidy between the wild groundnut species, Arachis, and cultivated genotypes hinder the transfer of useful alleles for agronomically important traits.
Iron and zinc grain concentrations diversity and agronomic performance of common bean germplasm collected from East Africa
This study evaluated 304 common bean lines sourced from the International Centre for Tropical Agriculture (CIAT) and its partners through the Pan Africa Bean Research Alliance (PABRA) for agronomic traits, disease response, yield, FESEED, and ZNSEED.
A Simple and Efficient Method for Extracting S. Rolfsii DNA For PCR Based Diversity Studies
This study describes a protocol for extracting high quality DNA from common bean using a DNA extraction buffer that contains sodium dodecyl sulphate and proteinase K to inactivate proteins and high salt concentration to precipitate the exopolysaccharides.
Chris Ojiewo, Theme leader – Genetic Gains Program, ICRISAT
Chris Ojiewo, Seed systems & Project coordinator for the Tropical Legumes projects, explains the many challenges involved in seed multiplication, why the private sector often fails to invest in these seeds, and what drives farmers to purchase seeds from the open market and not from authentic sources This video also explores what strategies are needed to improve seed supply and demand for the benefit of smallholder farmers.
Genome-wide discovery and deployment of insertions and deletions markers provided greater insights on species, genomes, and sections relationships in the genus Arachis
This study provides significant insights into the phylogenetic relationship among accessions, genomes, sub species and sections. These InDel markers are a useful resource for the groundnut research community – for genetic analysis and breeding applications.
